Coding

Part:BBa_K287004:Design

Designed by: Carlos Andres Sanchez   Group: iGEM09_IPOC1-Colombia   (2009-10-21)


Sodium Phosphate dependant channel


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1336
    Illegal PstI site found at 169
    Illegal PstI site found at 1887
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1336
    Illegal PstI site found at 169
    Illegal PstI site found at 1887
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1924
    Illegal BamHI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1336
    Illegal PstI site found at 169
    Illegal PstI site found at 1887
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1336
    Illegal PstI site found at 169
    Illegal PstI site found at 1887
    Illegal AgeI site found at 1177
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1674


Design Notes

The restriction sites were added by PCR with designed prymers. Restriction site at 5' XhoI, and 3' XhoI


Source

This part comes from a Genomic Sequence from the NCBI, syntethized by Stratagene

References